The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria.
Identifieur interne : 000028 ( Main/Exploration ); précédent : 000027; suivant : 000029The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria.
Auteurs : Angélique Chanal [France] ; Virginie Chapon ; Karim Benzerara ; Mohamed Barakat ; Richard Christen ; Wafa Achouak ; Frédéric Barras ; Thierry HeulinSource :
- Environmental microbiology [ 1462-2912 ] ; 2006.
Descripteurs français
- KwdFr :
- ADN bactérien (composition chimique), ADN bactérien (génétique), ADN des archées (composition chimique), ADN des archées (génétique), ADN ribosomique (composition chimique), ADN ribosomique (génétique), ARN ribosomique 16S (génétique), Alphaproteobacteria (MeSH), Analyse de séquence d'ADN (MeSH), Archéobactéries (classification), Archéobactéries (génétique), Archéobactéries (isolement et purification), Bactéries (classification), Bactéries (effets des radiations), Bactéries (génétique), Bactéries (isolement et purification), Biodiversité (MeSH), Climat désertique (MeSH), Dessiccation (MeSH), Données de séquences moléculaires (MeSH), Microbiologie du sol (MeSH), Phylogenèse (MeSH), Rayonnement ionisant (MeSH), Similitude de séquences d'acides nucléiques (MeSH), Tunisie (MeSH), Écosystème (MeSH).
- MESH :
- composition chimique : ADN bactérien, ADN des archées, ADN ribosomique, Archéobactéries, Bactéries.
- effets des radiations : Bactéries.
- génétique : ADN bactérien, ADN des archées, ADN ribosomique, ARN ribosomique 16S, Archéobactéries, Bactéries.
- isolement et purification : Archéobactéries, Bactéries.
- Alphaproteobacteria, Analyse de séquence d'ADN, Biodiversité, Climat désertique, Dessiccation, Données de séquences moléculaires, Microbiologie du sol, Phylogenèse, Rayonnement ionisant, Similitude de séquences d'acides nucléiques, Tunisie, Écosystème.
- Wicri :
- geographic : Tunisie.
English descriptors
- KwdEn :
- Alphaproteobacteria (MeSH), Archaea (classification), Archaea (genetics), Archaea (isolation & purification), Bacteria (classification), Bacteria (genetics), Bacteria (isolation & purification), Bacteria (radiation effects), Biodiversity (MeSH), DNA, Archaeal (chemistry), DNA, Archaeal (genetics), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Desert Climate (MeSH), Desiccation (MeSH), Ecosystem (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), RNA, Ribosomal, 16S (genetics), Radiation, Ionizing (MeSH), Sequence Analysis, DNA (MeSH), Sequence Homology, Nucleic Acid (MeSH), Soil Microbiology (MeSH), Tunisia (MeSH).
- MESH :
- chemical , chemistry : DNA, Archaeal, DNA, Bacterial, DNA, Ribosomal.
- geographic : Tunisia.
- classification : Archaea, Bacteria.
- genetics : Archaea, Bacteria, DNA, Archaeal, DNA, Bacterial, DNA, Ribosomal, RNA, Ribosomal, 16S.
- isolation & purification : Archaea, Bacteria.
- radiation effects : Bacteria.
- Alphaproteobacteria, Biodiversity, Desert Climate, Desiccation, Ecosystem, Molecular Sequence Data, Phylogeny, Radiation, Ionizing, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Soil Microbiology.
Abstract
The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and - molecular-based analysis. Thirty-one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria, Firmicutes, Proteobacteria and the CFB group while none related to Archaea. Analysis of 16S rRNA gene libraries revealed the presence of species related to Bacteria and Archaea. Sequences related to Archaea were all affiliated to the non-thermophilic Crenarchaeota subgroup. Bacterial sequences were dominated by Proteobacteria, Actinobacteria and Acidobacteria; a few sequences were distributed among eight others phyla, including Thermus/Deinococcus relatives. A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans. Because bacteria living in the hot desert of Tataouine are one way or another tolerant to desiccation, we investigate whether they could also be tolerant to radiation. Exposition of soil samples to intense gamma radiation yields Bacillus, Thermus/Deinococcus and alpha-Proteobacteria relatives. Four of these strains correspond to radiotolerant species as revealed by evaluation of the resistance levels of the individual cultures. A detailed analysis of the resistance levels for two Thermus/Deinococcus and two alpha-Proteobacteria relatives revealed that they correspond to new radiotolerant species.
DOI: 10.1111/j.1462-2920.2005.00921.x
PubMed: 16478457
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000797
- to stream PubMed, to step Curation: 000793
- to stream PubMed, to step Checkpoint: 000791
- to stream Main, to step Merge: 000028
- to stream Main, to step Curation: 000028
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria.</title><author><name sortKey="Chanal, Angelique" sort="Chanal, Angelique" uniqKey="Chanal A" first="Angélique" last="Chanal">Angélique Chanal</name><affiliation wicri:level="3"><nlm:affiliation>LEMIR, DEVM, DSV, UMR 6191 CNRS-CEA-Université Aix-Marseille II, IFR 112, CEA Cadarache, F-13108 Saint-Paul-lez-Durance, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LEMIR, DEVM, DSV, UMR 6191 CNRS-CEA-Université Aix-Marseille II, IFR 112, CEA Cadarache, F-13108 Saint-Paul-lez-Durance</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Saint-Paul-lez-Durance</settlement></placeName></affiliation></author><author><name sortKey="Chapon, Virginie" sort="Chapon, Virginie" uniqKey="Chapon V" first="Virginie" last="Chapon">Virginie Chapon</name></author><author><name sortKey="Benzerara, Karim" sort="Benzerara, Karim" uniqKey="Benzerara K" first="Karim" last="Benzerara">Karim Benzerara</name></author><author><name sortKey="Barakat, Mohamed" sort="Barakat, Mohamed" uniqKey="Barakat M" first="Mohamed" last="Barakat">Mohamed Barakat</name></author><author><name sortKey="Christen, Richard" sort="Christen, Richard" uniqKey="Christen R" first="Richard" last="Christen">Richard Christen</name></author><author><name sortKey="Achouak, Wafa" sort="Achouak, Wafa" uniqKey="Achouak W" first="Wafa" last="Achouak">Wafa Achouak</name></author><author><name sortKey="Barras, Frederic" sort="Barras, Frederic" uniqKey="Barras F" first="Frédéric" last="Barras">Frédéric Barras</name></author><author><name sortKey="Heulin, Thierry" sort="Heulin, Thierry" uniqKey="Heulin T" first="Thierry" last="Heulin">Thierry Heulin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16478457</idno><idno type="pmid">16478457</idno><idno type="doi">10.1111/j.1462-2920.2005.00921.x</idno><idno type="wicri:Area/PubMed/Corpus">000797</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000797</idno><idno type="wicri:Area/PubMed/Curation">000793</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000793</idno><idno type="wicri:Area/PubMed/Checkpoint">000791</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000791</idno><idno type="wicri:Area/Main/Merge">000028</idno><idno type="wicri:Area/Main/Curation">000028</idno><idno type="wicri:Area/Main/Exploration">000028</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria.</title><author><name sortKey="Chanal, Angelique" sort="Chanal, Angelique" uniqKey="Chanal A" first="Angélique" last="Chanal">Angélique Chanal</name><affiliation wicri:level="3"><nlm:affiliation>LEMIR, DEVM, DSV, UMR 6191 CNRS-CEA-Université Aix-Marseille II, IFR 112, CEA Cadarache, F-13108 Saint-Paul-lez-Durance, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LEMIR, DEVM, DSV, UMR 6191 CNRS-CEA-Université Aix-Marseille II, IFR 112, CEA Cadarache, F-13108 Saint-Paul-lez-Durance</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Saint-Paul-lez-Durance</settlement></placeName></affiliation></author><author><name sortKey="Chapon, Virginie" sort="Chapon, Virginie" uniqKey="Chapon V" first="Virginie" last="Chapon">Virginie Chapon</name></author><author><name sortKey="Benzerara, Karim" sort="Benzerara, Karim" uniqKey="Benzerara K" first="Karim" last="Benzerara">Karim Benzerara</name></author><author><name sortKey="Barakat, Mohamed" sort="Barakat, Mohamed" uniqKey="Barakat M" first="Mohamed" last="Barakat">Mohamed Barakat</name></author><author><name sortKey="Christen, Richard" sort="Christen, Richard" uniqKey="Christen R" first="Richard" last="Christen">Richard Christen</name></author><author><name sortKey="Achouak, Wafa" sort="Achouak, Wafa" uniqKey="Achouak W" first="Wafa" last="Achouak">Wafa Achouak</name></author><author><name sortKey="Barras, Frederic" sort="Barras, Frederic" uniqKey="Barras F" first="Frédéric" last="Barras">Frédéric Barras</name></author><author><name sortKey="Heulin, Thierry" sort="Heulin, Thierry" uniqKey="Heulin T" first="Thierry" last="Heulin">Thierry Heulin</name></author></analytic><series><title level="j">Environmental microbiology</title><idno type="ISSN">1462-2912</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alphaproteobacteria (MeSH)</term><term>Archaea (classification)</term><term>Archaea (genetics)</term><term>Archaea (isolation & purification)</term><term>Bacteria (classification)</term><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>Bacteria (radiation effects)</term><term>Biodiversity (MeSH)</term><term>DNA, Archaeal (chemistry)</term><term>DNA, Archaeal (genetics)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (genetics)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Desert Climate (MeSH)</term><term>Desiccation (MeSH)</term><term>Ecosystem (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Radiation, Ionizing (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Soil Microbiology (MeSH)</term><term>Tunisia (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (composition chimique)</term><term>ADN bactérien (génétique)</term><term>ADN des archées (composition chimique)</term><term>ADN des archées (génétique)</term><term>ADN ribosomique (composition chimique)</term><term>ADN ribosomique (génétique)</term><term>ARN ribosomique 16S (génétique)</term><term>Alphaproteobacteria (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Archéobactéries (classification)</term><term>Archéobactéries (génétique)</term><term>Archéobactéries (isolement et purification)</term><term>Bactéries (classification)</term><term>Bactéries (effets des radiations)</term><term>Bactéries (génétique)</term><term>Bactéries (isolement et purification)</term><term>Biodiversité (MeSH)</term><term>Climat désertique (MeSH)</term><term>Dessiccation (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Microbiologie du sol (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Rayonnement ionisant (MeSH)</term><term>Similitude de séquences d'acides nucléiques (MeSH)</term><term>Tunisie (MeSH)</term><term>Écosystème (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Archaeal</term><term>DNA, Bacterial</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Archaea</term><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN bactérien</term><term>ADN des archées</term><term>ADN ribosomique</term><term>Archéobactéries</term><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Archaea</term><term>Bacteria</term><term>DNA, Archaeal</term><term>DNA, Bacterial</term><term>DNA, Ribosomal</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>ADN des archées</term><term>ADN ribosomique</term><term>ARN ribosomique 16S</term><term>Archéobactéries</term><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Archaea</term><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Archéobactéries</term><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alphaproteobacteria</term><term>Biodiversity</term><term>Desert Climate</term><term>Desiccation</term><term>Ecosystem</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Radiation, Ionizing</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Nucleic Acid</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alphaproteobacteria</term><term>Analyse de séquence d'ADN</term><term>Biodiversité</term><term>Climat désertique</term><term>Dessiccation</term><term>Données de séquences moléculaires</term><term>Microbiologie du sol</term><term>Phylogenèse</term><term>Rayonnement ionisant</term><term>Similitude de séquences d'acides nucléiques</term><term>Tunisie</term><term>Écosystème</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and - molecular-based analysis. Thirty-one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria, Firmicutes, Proteobacteria and the CFB group while none related to Archaea. Analysis of 16S rRNA gene libraries revealed the presence of species related to Bacteria and Archaea. Sequences related to Archaea were all affiliated to the non-thermophilic Crenarchaeota subgroup. Bacterial sequences were dominated by Proteobacteria, Actinobacteria and Acidobacteria; a few sequences were distributed among eight others phyla, including Thermus/Deinococcus relatives. A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans. Because bacteria living in the hot desert of Tataouine are one way or another tolerant to desiccation, we investigate whether they could also be tolerant to radiation. Exposition of soil samples to intense gamma radiation yields Bacillus, Thermus/Deinococcus and alpha-Proteobacteria relatives. Four of these strains correspond to radiotolerant species as revealed by evaluation of the resistance levels of the individual cultures. A detailed analysis of the resistance levels for two Thermus/Deinococcus and two alpha-Proteobacteria relatives revealed that they correspond to new radiotolerant species.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Saint-Paul-lez-Durance</li></settlement></list><tree><noCountry><name sortKey="Achouak, Wafa" sort="Achouak, Wafa" uniqKey="Achouak W" first="Wafa" last="Achouak">Wafa Achouak</name><name sortKey="Barakat, Mohamed" sort="Barakat, Mohamed" uniqKey="Barakat M" first="Mohamed" last="Barakat">Mohamed Barakat</name><name sortKey="Barras, Frederic" sort="Barras, Frederic" uniqKey="Barras F" first="Frédéric" last="Barras">Frédéric Barras</name><name sortKey="Benzerara, Karim" sort="Benzerara, Karim" uniqKey="Benzerara K" first="Karim" last="Benzerara">Karim Benzerara</name><name sortKey="Chapon, Virginie" sort="Chapon, Virginie" uniqKey="Chapon V" first="Virginie" last="Chapon">Virginie Chapon</name><name sortKey="Christen, Richard" sort="Christen, Richard" uniqKey="Christen R" first="Richard" last="Christen">Richard Christen</name><name sortKey="Heulin, Thierry" sort="Heulin, Thierry" uniqKey="Heulin T" first="Thierry" last="Heulin">Thierry Heulin</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Chanal, Angelique" sort="Chanal, Angelique" uniqKey="Chanal A" first="Angélique" last="Chanal">Angélique Chanal</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000028 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000028 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16478457
|texte= The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16478457" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |